![]() A wet transfer, on the other hand, is ideal for routine protein work and most flexible in terms of blotting times, voltage settings and cooling options. Semi-dry transfer systems are easier to set up, take up less time and require less buffer. The Tricine system requires SDS in sample and running buffers for best results. A semi-dry transfer is suitable for convenience and speed provides high output results. Designed for rapid 510 minute semi-dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes, the Invitrogen Power Blotter utilizes. Our Tricine gels have a 4 stacking gel and do not contain SDS. The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional Towbin buffer. Formulation Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. The Power Blotter System achieves highly efficient transfer in seven minutes when used with Power Blotter Select Transfer Stacks or ten minutes with Power Blotter 1-Step Transfer Buffer. The easy-touch programming feature allows custom transfer settings to be quickly created, saved, and run. The included Power Blotter Station has an easy-to-use color LCD touchscreen interface with pre-programmed transfer methods for different numbers and sizes of gels and ranges of molecular weight proteins. The Power Blotter XL Cassette, included in the Power Blotter XL System, enables the simultaneous transfer of up to four mini-size or two midi-size gels. Assemble iBlot 2 Transfer Stacks on the iBlot 2 Gel Transfer. Perform blotting within 15 minutes of assembling the stacks with the gel. Learn more about the semi-dry transfer device: Power Blotter XL System Close and latch the lid of the device carefully to ensure that the Transfer Stack and electrical contacts do not shift. Versatile-compatible with Power Blotter Select Transfer Stacks and Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods.Flexible-simultaneous transfer of 1 to 4 mini-size or 1 to 2 midi-size gels with the Power Blotter Cassette XL After electrophoresis, gels were transferred to nitrocellulose membrane using either the Fast Semi-Dry Transfer System or the Invitrogen iBlot Dry.Easy-touch programming-convenient pre-programmed transfer methods or create, save, and run customized transfer methods.Integrated power supply-seamless operation between control unit and cassette no need for external power supply.Fast-5- to 12-minute transfer when used with Power Blotter Select Transfer Stacks or Power Blotter 1-Step Transfer Buffer.Efficient-high transfer efficiency with a broad range of protein sizes compared to conventional semi-dry or wet (tank) transfer methods.The Power Blotter System includes an integrated power supply optimized to enable consistent and high-efficiency protein transfer.įeatures of the Power Blotter XL System include: ![]() ![]() The Invitrogen Power Blotter XL System is specifically designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes in 5 to 12 minutes. ![]()
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